Briefly, RNAProtect bacterial reagent (Qiagen, Valencia, CA) was added according to the manufacturer's instructions to the cultures grown to stationary phase; treated cells were stored at -80°C (for no longer than 24 h) until RNA isolation was performed.
CR-RT-PCR was performed with 20 μg of total RNA prepared from P. abyssi cells in entry into stationary phase, treated with or without 25U of Tobacco Acid Pyrophosphatase TAPP) according to manufacturer's protocol (Epicentre Biotechnologies).
The period until standard deviation of split frequencies for the two independent tree samples fell below 0.01 (indicating convergence to the stationary distribution) was treated as burn-in.
In cells grown to stationary phase or treated with rapamycin, the amounts of Pol I Rrn3p complexes are significantly reduced (Milkereit & Tschochner, 1998; Claypool et al., 2004).
The B. burgdorferi stationary-phase cells treated by daptomycin had almost all red fluorescence as spirochetes.
Microscopic examination determined that the round-body forms were the majority of cells in cefoperazone treated stationary-phase B. burgdorferi cultures.
It is shown that the contact force can be treated as a nonzero mean stationary process with a normal distribution.
These two shocks can be treated as stationary under this assumption.
Due to good thermal conductivity of structural metal materials, low heat capacity and a sufficiently long time of irradiation the task was treated as stationary.
The data for mortality come from a demographic projection until 2060 and are subsequently treated as stationary until the final steady state (see Section 4).
