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Considering the LC50, sublethal 96-h static exposures were designed for O. hatcheri (0.1 0.5 μg L−1 AZM) and J. multidentata (5 10 μg L−1AZM) to determine biochemical endpoints.
WAF was diluted with system water to 1 2, 1 5, 1 10, and 1 100, and static exposures were performed in 30 mm glass Petri dishes (25 embryos in 4 mL) in an incubator at 28.5°C.
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After 72 h of static exposure, algal cell density was determined by measuring fluorescence (Multiple Plate Reader Tecan ULTRA).
If, for example, the EC50 value of a combination product for the endpoint "algal growth inhibition" was derived in a test with D. subspicatus and based on biomass measurement after 3 days of static exposure, EC50 value for the a.s.s
For their use in water monitoring, the typical physico-chemical characteristics of the samplers, i.e. the mass-transfer coefficient (k0) and the sampler-water partition coefficient (Ksw), were determined based on a static exposure design.
The fish were exposed to the SWCNTs based on a static exposure regime.
The fish were exposed to the AgNMs based on a static exposure regime.
Following the 24 h static exposure, the water from each jar was gently decanted and each crab was carefully removed.
Compared with exposures initiated at 5.25 hpf (50% epiboly), static exposure to 3 μM TDCPP at 0.75 96 hpf resulted in a significant increase in mortality and developmental abnormalities.
Figure 3 shows representative images of the relative severity of these phenotypes following static exposure to 3 μM TDCPP during 0.75 96 hpf.
Static exposure to ≥ 8 µM TDCPP during 5.25 96 hpf resulted in a significant increase in mortality (LC50 = 8.5 µM) compared with vehicle controls.
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