The Gators forced a Ball State turnover and went on to a 40-19 victory.
The only Oregon State turnover turned out to be the difference.
Steady-state turnover measurement results indicated that Gox2181-D43Q couse use both NADH and NADPH as its coenzyme, and so could Gox2181-Q20R&D43Q.
Pre-steady-state turnover rates for APE1's endonuclease activity have been estimated to be greater than 700 or 850 s–1, whereas steady-state turnover rates are approximately 2 s–1.
The weakness of this selection results in a steady-state turnover of very slightly deleterious and beneficial substitutions.
However, variants BsDHFR-L20M and BsDHFR-L20M/A104Q show an almost 2-fold decrease in the steady-state turnover numbers.
The steady-state turnover numbers of C cO in whole cells under uncoupled conditions are shown in Figure 2(A).
From these data we conclude that water dissociation is rate-limiting for steady state turnover in mutant Q228E.
More insightful is the inspection of the steady-state turnover number, kcat, and the Michaelis constant, KM.
This correspondence indicates that the rate-limiting transition state for enzyme-DNA association and steady-state turnover under limiting substrate conditions share similar electrostatic characteristics.
