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All centres completed the required assays and returned the raw data to the organising centre for central analysis, which included all replicates unless specifically stated (Supplementary MIATA Information, ELISPOT and Multimer Module 4A).
Of those trials with RT, 68% of the sponsorship was academic, 23% was from industry, and 9% was combined; whereas for the trials without RT, 48% was academic, 39% was from industry, and 12% not stated (Supplementary Table S1).
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All the strains used in this study are in the W303 genetic background and are RAD5+, unless otherwise stated in Supplementary Table 1.
Similar results were obtained with antibodies to H4 acetylated at lysine 16 (H4K16againagain with no correspondence between acetylation and transcriptional state (supplementary Fig. S1).
Our results clearly detected the change in the heterozygous state (Supplementary Table 7).
The staining of pStat5 was rapidly lost upon entering the involution state (Supplementary Figure S4).
In fact, the P3 region also exists as a random coil in the free state (Supplementary Figure S5).
FGF21 deficiency had no effect on the production of GH in either fasting or fed state (Supplementary Figure S4A).
Similarly, in the structure of MuPPT, Glu157 is found in a different rotameric state (Supplementary Figure 8).
After fusion, the tetraploid nucleus undergoes concerted chromosome loss to regenerate the diploid state (Supplementary Fig. 2B).
In the normal human pancreas, these cells are present in an inactive α-SMA-negative state (Supplementary Fig S1A).
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