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The general method used to construct Bacillus lysogens (unless otherwise stated) required mid-exponential phase cultures (grown in 5 ml BHI at 30°C with aeration at 150 rpm in 50 ml FalconTM tubes) to be infected with 1×108 phage particles for 30 minutes, washed, and plated (undiluted and three 100-fold serial dilutions thereof) on BHI agar for 16 hours at 30°C.
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