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This suggests that growth factors and nutrient starvation use two different signaling pathways that converge at the level of Mcl-1 degradation to induce apoptosis.
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As the pressure mounted to provide the all-powerful state with more and more output, the use of extreme violence became the norm, with starvation used as a weapon to punish those who could not keep up with the work routine demanded of them.
After overnight culturing, the cells were subject to 24 hrs starvation using the serum-free medium.
We tested the mutant (1F8) and complemented (1F8 + pCNAG_07701) strains for sensitivity to copper starvation using the copper chelator bathocuproinedisulfonic acid (BCS).
We hypothesized that accumulated ROS cause loss of mtDNA in atg mutant cells, and investigated ROS accumulation during nitrogen starvation using dihydroethidium (DHE).
In this study, we investigated the transcriptional response to potassium starvation using Serial Analysis of Gene Expression (SAGE -tag SAGE -tagg.
We constructed a mathematical model of weight loss under total starvation using the established principles of energy balance.
This was probably due to the specific conditions of alcoholic stress and nutrient starvation used in our study.
DOI: http://dx.doi.org/10.7554/eLife.05289.003 We next evaluated whether endogenous Beclin 1 undergoes S90 phosphorylation in response to nutrient (amino acid) starvation using the Beclin 1 S90 phosphospecific antibody.
First, we determined the subcellular localization of the master protease Pep4-GFP and Prc1/CPY-RFP in ESCRT mutants under rich growth or starvation using live cell fluorescence microscopy.
Reviewer #2: This study examines the intracellular fate of two key components of yeast TORC1, namely, Tor1 and Kog1, following nutrient starvation using fluorescence microscopy.
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