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The measured magnitude of regulation was rather small for the majority of these genes (<∼2-fold), perhaps due to the incomplete synchronization and variability inherent in the starvation method, or the limited sensitivity of oligo-based microarray detection.
Another important quality factor is the starvation method.
Candidate recombinants, including RR4108, were reassayed with the MIV starvation method.
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Some members even organize pro-ana games where members compete with each other to lose weight, collecting points for obeying various self-starvation methods.
PC formation can be induced in vitro in both fibroblasts and RPE1 cells, a widely used model to study ciliogenesis, by growing cells to confluence and this process can be enhanced upon serum starvation (see methods).
For example, humanitarian law rules do prohibit the use of starvation as a method of warfare and the targeting of essential resources (being classed as civilian objects),12 thereby providing limited protection of materials.
Our data set suggests that caution is warranted if the intention is to use serum starvation as a method to draw conclusions about protein abundance variations that occur in a normal, unperturbed, proliferating cell cycle.
The use of serum-starvation as a method to induce monocyte apoptosis in vitro is convenient and provides consistent results.
Cell populations can be synchronized at specific stages of the cell cycle through various means including serum starvation and chemical methods.
Consistent with low tph-1 expression in the absence of food, serotonin levels were reduced in NSM and ADF after starvation ('Materials and methods' and Liang et al., 2006).
To allow sufficient time for recB strains, which grow slowly, to exit stationary phase, these were incubated in growth medium for an additional 30 min prior to thymine starvation (Materials and Methods).
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