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We report the regulatory response of C. crescentus to carbon starvation, based on combined high-throughput proteome and transcriptome analyses.
We chose a time period of 2.5 hours to eliminate the effect of starvation based on a preliminary study where we examined the effect of starvation, hypoxia and a combination of both (unpublished observations).
We have built a qualitative logical model of nutrient starvation based on Petri nets.
In addition, our results confirm and extend the importance of autophagy genes and vacuolar degradation in mitotic exit during nitrogen starvation; based on analysis of spindle morphology, we found that vps30Δ/ atg7∆, and7∆, and pep4∆ yeasts arrest in telophase.
We will first generate a mathematical model of the process of starvation based on our previous data concerning energy demands and body composition in a population of 592 adults (Speakman and Westerterp, 2010).
- Although Matsui et al. conclude that yeast TORC1 may be dispensable for cell cycle progression during nitrogen starvation (based on the lack of an observed effect of rapamycin in wild-type yeasts), it is notable that rapamycin does not suppress mammalian MTORC1 serine 2481 phosphorylation which enriches MTORC1 near the contractile ring during telophase and cytokinesis.
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In contrast, a transcriptional signal of P-starvation was not apparent despite evidence of P-starvation based on their rapid growth response to P-addition.
Thus, although the rapid growth response following the addition of PO4 indicates P-starvation in these cells, the transcriptional profile was not informative of P-starvation based on these phosphorus transport and metabolism genes.
A period of starvation is, based on earlier results, predicted to reduce longevity but to leave cold tolerance unaffected ([ 23, 36, 37]; see Table 1 for specific hypotheses).
IN THE 200 years since Thomas Malthus forecast that population growth would result in mass starvation, dire predictions based on demographic trends have come to be taken with a pinch of salt.
As with the starvation-induced dataset, based on Monte Carlo simulations, the increased number of MMS-induced genes that map near ORC binding sites compared to all genes is statistically significant (p < 10-2).
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