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Furthermore, comparing human PEG10 with PEG10 from other species, translation of the orthologous proteins starts upstream of the predicted human AUG.
The homology starts upstream from the 5'-end of OsRpl6-1 cDNA (Fig. 4A, bent arrow) and extends into the 5'-part of the intron.
Polycistronic RNA initiating from H1 starts upstream of MT-TF, the gene encoding mt-tRNAPhe and has a defined termination site within MT-TL1 (mt-tRNALeu UUR)).
Second, one these putative promoters yield transcript that starts upstream of the rho-independent terminator sequence (Fig. 1b, c) that may prematurely terminate claR transcription.
However, it should be pointed out that digestion of ABPV capsid protein VP1 with protease V8 revealed a long peptide in the N-terminal region of VP1, indicating that initiation of translation starts upstream of the glutamate codon at position 14 (Fig. 1D).
On the other hand, the planning and scheduling of interventions within an operating room area, is not a simple allocation of space and time that is assigned to surgical teams on a given day, but a complex process that starts upstream with the management of a waiting list, where the patients to schedule are queuing until there is an allocated date, and a time and a room in the OR theatre.
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That's why we start upstream.
Instead of the usual method of getting into the wave — starting upstream and allowing the current to draw me into place — I would start downstream from the wave lying flat on the board, and use a rope to counter the river's flow, swinging out into position, popping up into a surf stance, and then making my way into the wave.
An RNA start upstream of the canonical EBER1 start is used to a minor extent in the transgenic lines 127 and 131 (20% or less) as was described for the EBV positive BL cell line Raji [27], [28] (Figure 3C, panel iii and 3D, panel v).
Therefore, we developed a qRT-PCR strategy to quantify transcripts that started upstream of the canonical TSS for IRF1.
Starting upstream, perhaps one lesson is to increase our attention to prevention of complications that render one susceptible to a prolonged critical illness.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com