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Our investigations reveal the predominantly used transcription start sites (TSSs) for each gene including novel transcription start sites for FOXG1.
Transcription start sites for each gene selected for detailed promoter analysis were obtained using the database of transcription start sites version 6.0 [ 21].
Arrow indicates transcription starting site.
The minimum distance by default for both the locus start site to ORF start site and for the locus start site to ORF end site was −65.
For the purpose of this study, the one kilobase (kb) region upstream of the annotated transcription start site for each gene was evaluated for promoter motifs.
The transcription start site for miR-31 was identified.
The highest-ranked aligned start site was then chosen as the new start site for the set of orthologs.
Gene groups were also evaluated for biological relationship by searching for shared transcription factor binding sites (TFBSs) within 1 kb upstream of the transcription start sites of individual genes using Genomatix Gems Launcher [ 27], with a level of significance that includes FDR correction.
We have found evidence for non-canonical translation start sites.
It was recently evaluated that in mouse transcriptome there were about 1.32 5' start sites for each 3'-end (The FANTOM consortium et al. The transcriptional landscape of the mammalian genome. Science. 2005. 309. 1559 1563).
We evaluated for enrichment by considering binding sites that were located up to 700 bp upstream or 300 bp downstream of all the transcription start sites reported in the GENCODE database and that had a score larger than 750.
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