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However, identical observation data is retained at every time step, and there will the same starting quantity of particles N for every filter.
Amphioxus β-actin was used as control to normalize the starting quantity of RNA.
The five dilutions used in the standard curve to calculate amplification efficiency were run in triplicate and were serially diluted two-fold from a starting quantity of 20 ng/µl.
All qPCR assays were standardized to starting quantity of RNA.
Therefore, a higher Ct value indicates a lower starting quantity of nucleic acid.
As previously reported, the Ct value correlates to the starting quantity of target mRNA [ 25].
Similar(33)
Each BeadChip was run with samples from two starting quantities of RNA (three samples from both chosen starting quantities), and each possible combination of the two starting quantities was run once and only once amongst the six BeadChips.
Data were analyzed using the iCycler Software, and starting quantities of message levels of each gene were determined from constructed standard curves.
The assay used lower starting quantities of total RNA because RNA was limiting (recommended 5-8 μg).
It is clear that all things being equal, of the range of starting quantities of RNA considered here, it is preferable to use 250 ng.
Average cycle threshold values for experimental samples were compared with a standard curve of genomic DNA from strain BG2389 to generate relative starting quantities of DNA.
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