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For beads-scavenging experiments, 20 μl 2 μm TRITC-labeled beads were injected intravenously 5 min after starting imaging.
The elapsed time (s) after starting imaging of an embryo is indicated at the left upper corner.
At 24 h after an overdose of APAP, mice were intravenously injected with sets of fluorochromes before starting imaging.
After liquefaction, spermatozoa were prepared by direct swim-up and allowed to capacitate in a supplemented Earle's balanced salt solution (sEBSS) containing 1.8 mM CaCl2, 5.4 mM KCl, 0.81 mM MgSO4, 25.0 mM NaHCO3, 1.0 mM NaH2PO4, 116.4 mM NaCl, 5.5 mM d-glucose, 2.5 mM Na pyruvate, 41.8 mM Na lactate and 0.3% (w/v) BSA, for at least 3 h at 37°C under 5%CO2/95%% air before starting imaging.
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Similar to the method described for CTA, a test bolus method can be performed, administering 1-2 ml of GBCA and acquiring a series of rapid two-dimensional (2D) images of the vessel in question to determine the optimum time to start imaging post injection.
"Once the containers get on site, we plug in power, water, network connectivity, and the boxes inside wake up, figure out which property group they belong to and start imaging themselves.
Soon, we will give a build of Windows 10 to our OEM partners so they can start imaging new devices with Windows 10.
Huerta also calls attention to fluorescent resonance energy transfer, which he says, "really allowed us to start imaging processes in cells as they occur".
More recently, scientists have started imaging the brains of teenagers before and after they start using marijuana, but the research needs to continue for much longer to fully understand how cannabis affects the developing brain.
Soon after we started imaging the night-side of Venus with IR2, a significant amount of light was found to be spreading from the dayside disk, contaminating pixels far from the source.
Formal entry into service is expected in about three months' time, although it is likely that the platform will start imaging the Earth as early as next week to begin the process of instrument calibration.
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