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The animals were allowed a period of 2 3 weeks to recover from surgery before starting acquisition of any physiological data.
They were then placed on imaging plates containing 0.5 μg/ml doxycycline for 1 hr at 38°C before starting acquisition.
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Trial Control settings were configured to start acquisition after the subject was detected within the arena for less than 1 s.
The probe allows starting the acquisition of the signal about 5ms after the end of the excitation pulse.
Injection needles were preloaded with adequate solutions and collocated in subcutaneous, intraperitoneal or intramuscular regions before starting image acquisition.
The hit of the hammer was used as trigger for starting trial acquisition.
Framing, focusing, and starting image acquisition, in that order, were performed within 20 sec of sample rotation.
The water in the microchamber was then rapidly replaced by 100 μl of the treatment solution before starting image acquisition.
Linear phase encoding was used, resulting in the maximum signal being detected 5 min after starting the acquisition.
The objective has been, from the start, acquisition of that nation's resources.
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