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In view of the developmental changes during culture, we started treatments for the different groups at different times and harvested cells at the same age of cultured neurons (e.g., Ctl, no ethanol treatment; CIE, starting ethanol treatment on DIV 8; CIEW2, on DIV 6; and CIEW5, on DIV 3).
We attribute the lack of significant responses in soil respiration to the experimental treatments during the first year to a combination of two factors: firstly we started treatments during the dry season when decomposition is limited by the lack of moisture [23] and secondly, we did not include the litter layer in our measurements of soil respiration.
Mice started treatments with intravesical VEGF (or PBS in the control group) 10 days after labeling of bladder sensory neurons.
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Gray had just started treatment with a psychologist at the time of the offence.
So I started treatment.
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Donna started treatment right away the morning after we arrived.
Rita was able to get medical assistance and started treatment by the end of the week.
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