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He currently only mentors a handful of people but has started to amplify his message to his audience, and 60,000+ member group he has assembled.
When Peff was determined, the relative difference between the libraries was calculated from the average cycle thresholds where the 107 diluted triplicates started to amplify.
The average primer efficiency (Peff) was calculated based on the 10-fold molecule difference of the dilutions of the libraries and the difference of cycle threshold (Ct) the samples started to amplify.
( <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0010029.e001.PNG" class= inline-graphic"/>, where cA and cB are the concentrations of sample A and sample B, respectively; and correspondingly CtA and CtB are the cycle times when A and B started to amplify).
When injected, Levovist started to amplify the Doppler signal with a mean delay of about 15 s (20 120 s) after injection with persistence of enhancement for approximately 8 min (3 15 min).
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Sonically, we're not going to compete with pop unless we start to amplify, which would defeat the purpose.
This could mean that Earth gets even hotter as the land starts to amplify human-induced warming rather than slowing it down.
Once you've started to become unnerved, amplify the effect by putting yourself into a paranoid mindset.
He says that many people, including professors, told him he was wasting his time when he first started trying to amplify waves of radiation into a continuous stream.
More recently, immunologist started to explore the possibility to amplify patient's immune response against tumours using vaccination.
Classically this is achieved in a more or less random manner or by making a small number of specific changes to an existing sequence (see below); however, with the emergence of 'synthetic biology' a greater diversity of sequences can be created by assembling the desired sequence de novo (without a starting template to amplify from).
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CEO of Professional Science Editing for Scientists @ prosciediting.com