Under our conditions, long lasting currents showed no fast inactivation, started to activate at −50 mV, reached a peak at 100 mV, exhibited a reversal potential of about +140 mV and a maximal conductance of 280 nS according to the mean current-voltage relationship (Figure 3a; b, n = 11, error bars denote s.e.m).m
"Hopefully if I can get the job then I start to activate that".
Once a stimulus s and context c are activated, the agent starts to activate the internal processes as mentioned above and once the agent performed the action, a mechanism is assumed that stops the stimuli as an action effect: the agent has performed the task and due to that environment has changed.
Moreover, the narrow spacing of dense bands of defects and/or dislocations along the basal planes near GaN surface suggests that, in the later stage of indentation, a larger indentation load, such as the 100 mN applied in the present work, starts to activate the extensive slip bands along the ∼60° pyramidal planes.
This is starting to activate things here in lower Manhattan".
However, if treatment is prolonged, glioblastomas start to activate new pathways which may induce drug resistance.
As p53 level goes above the KMc threshold, p53 starts to activate the expression of gene MDM2 and thus the production of cytoplasmic Mdm2.
Typical for heterologously expressed SCN5A current measured with square form VC steps, current starts to activate around −60 mV, peaks around −30 mV, and subsequently decreases in amplitude due to the reduction in Na+ driving force (Fig. 1b). Figure 1c shows averaged data for the voltage-dependence of 'steady-state' activation.
The amplification conditions were; a 10 min hot start to activate the polymerase followed by 50 cycles of 95°C for 15 sec and 60°C for 1 min. Amplification specificity was confirmed by direct sequencing of the amplicons.
The PCR was started at 95°C for 15 minutes (hot start) to activate the AmpliTaq polymerase, followed by a 45-cycle amplification (Denaturation at 94°C for 20 seconds, annealing at 60°C for 30 seconds, extension at 72°C for 60 seconds, and plate reading at 60°C for 10 seconds).
Amplification conditions were; a 10 min hot start to activate the polymerase followed by up to 50 cycles of 95°C for 15 sec and 60°C for 1 min. The number of cycles required for the fluorescence to become significantly higher than background fluorescence (termed cycle threshold [Ct]) was used as a measure of abundance.
