They received the same 15 min instruction by an experienced observer (FMA) before they started the measurements.
We have started the measurements of samples of callus obtained in the course of surgical revisions of slow healing bone fractures.
Still, due to poor condition of the school, some corrective measures were performed before starting the measurement of consumption.
The importance of achieving thermal equilibrium on the sample before starting the measurement is emphasised.
A technician taps a green arrow on a digital screen on the device to start the measurement.
One minute after starting the measurement, TRH (33 µM) or taltirelin (33 µM, 150 µM for single-cell response) were administered for 15 s.
After starting the measurement, the run proceeded and terminated automatically.
After detecting successfully, the subject can start the measurement.
A zero calibration was performed before starting the measurement phase of the protocol.
Sample was outgassed under primary vacuum for 24 h at 120 °C before starting the measurement.
