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Sepsis was induced (ip injection of faecal slurry), and fluid resuscitation (10 ml/kg/h) started 2 hours later.
Ringer's perfusion (3 µl/min) was started 2 hours before the start of the 2-h drug infusion period and lasted for 2 more h after the change back to Ringer = s.
Remarkably, the increase in glucose uptake started 2 hours after LPS injection, earlier than other organs.
Treatment started 2 hours before induction of AIA and was continued every 24 hours for the indicated time periods (3, 21, or 42 days).
The nebulizations of 5,000 IE heparin every 4 hours were started 2 hours post-injury and 2 mg tPA were nebulized every 4 hours, starting 4 hours post-injury.
Animals randomly received vehicle (control, n = 14) or the intravenous H2S donor Na2S started 2 hours before hemorrhage (pre-treatment, n = 11), at the beginning of blood removal (early post-treatment, n = 10) or at the beginning of resuscitation (late post-treatment, n = 10).
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Imaging started 6 hours after addition of BMP4.
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The trip started 12 hours before in Mazatlán, Sinaloa.
Play started 3 hours 25 minutes late because of fog, with many players unable to finish before dark. .
Selection was started 4 hours after nucleofection.
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