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During the start-up course, the granular sludge experienced a process of initial disintegration and subsequent reaggregation.
On day 45, in the start-up course, distinct crack appeared along with the generation of sediment, which has not been mentioned in the anammox literature seen).
Soon after the addition, anammox reaction appeared and the nitrogen removal efficiency enhanced continuously, suggesting that the start-up course turned into activity elevation phase (104 148 d).
During the start-up course, the granular sludge initially disintegrated and then reaggregated and turned red, suggesting the high anammox performance.
During the start-up course, the anaerobic granular sludge can be directly converted into red anammox granules, which plays a crucial role in stable and efficient operation of anammox reactor.
However, the subsequent increase of nitrite concentration after reaching 252 mg/L no longer improved the volumetric substrate nitrogen removal rate (149 164 d), the function of anammox became close to saturation, and the start-up course came into the stationary phase [ 12].
Among researches on anammox start-up course seeded by various conventional sludges [ 11, 16– 23], anaerobic granular sludge has been reported as a preferable inoculum based on compact structure with very good settling properties, high removal efficiency, and strong shock load capacity [ 24].
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