Sentence examples for start the assay from inspiring English sources

Of 4 patients, not enough cells were retrieved to start the assay.

20 mM succinate and mitochondria were added to the assay buffer, and incubated for 10 minutes at 30°C, followed by the addition of 5 mM MgCl2, 2 mM KCN, 5 µg/ml antimycin A, 5 µg/ml rotenone, 50 µM DCPIP and finally 65 µM ubiquinone-1 to start the assay.

ACC oxidase assay cofactors were added and ACC was used to start the assay.

The cells were warmed to 35°C to start the assay.

The PM consists of two components, which are combined to start the assay.

The serum vials were sealed with aluminum crimps and warmed for 5 min at 35°C to start the assay.

Before starting the assay, samples were incubated in 100mmol/L phosphate buffer (pH 7.5) for 1 hour at 37°C to inactivate non-cathepsin S activity, after which the pH was returned to pH 6.0 using 0.5mol/L of MES buffer.

Replacing serum-free medium in the lower chamber with 10% FBS-containing medium started the assay.

B. Before starting the assay Warm up the water bath and a drying chamber/incubator to 37°C.

Instead of starting the assay with a 25 °C device, the enrichment assay was carried out with a device that was maintained at 39 °C constantly.

Using this system, we could focus the effect on network stability or maturation, because network formation of HUVECs was complete at the time we started the assay.