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The dilution of the purified enzyme or membrane to start reactions was >100-fold.
To each reaction, 0.2 μg/μl bacterially expressed recombinant GST-Eg5 was added, and to start reactions, 10 μl of 100 μM ATP (made up in kinase assay buffer) was added.
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Sample extracts were incubated with the enzyme in the buffer for 15 min before adding substrate to start reaction.
These two different starting reactions are shown in Figure 3.
PET is produced by step-growth polycondensation from terephthalic acid (TPA) and ethylene glycol (EG), and the synthesis of PET involves two different starting reactions.
According to these observations, starting reaction between reactants (1) and (2) is the more rapidly occurring reaction amongst competing reactions (see step1, Fig. 10).
We designed a facile thermal conversion route to synthesize 2H-WS2 nanoflakes using different tungsten oxide nanorods and S powder as starting reaction reagents.
The eluted material contained a mixture of GDP and non-exchanged ppGpp but no GMPPNP was detected despite its 10-fold excess to GDP in the starting reaction.
The above three enzymes have the ability to complete hydrolysis of camel lactoferrin after 0.5 and 1.0 h from the starting reaction at the 1 50 ratio.
I'm starting to get reactions to today's column.
As shown in Fig. 6, the start reaction temperature of the composite material is about 450 °C, and the oxidation reaction of the graphene oxide is completed at 600 °C.
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