Sentence examples for start quantity from inspiring English sources

Alpha and beta globin mRNA were reduced from a portion of the total RNA samples using the GLOBINclear™ Human kit (Ambion, Austin, TX, USA) according to the manufacturer's instructions with the recommended start quantity of 2 μg of total RNA.

However, identical observation data is retained at every time step, and there will the same starting quantity of particles N for every filter.

Amphioxus β-actin was used as control to normalize the starting quantity of RNA.

standard curves for each primer set were generated by varying starting quantity between 300 and 300,000 total copies.

200ng of Total RNA was used as the starting quantity for amplification and labelling of all samples.

The housekeeping gene hypoxanthine phosphoribosyl transferase (HPRT, Applied Biosystems; Hs99999909_m1) was used as an endogenous control to normalize RNA starting quantity.

The five dilutions used in the standard curve to calculate amplification efficiency were run in triplicate and were serially diluted two-fold from a starting quantity of 20 ng/µl.

All qPCR assays were standardized to starting quantity of RNA.

Therefore, a higher Ct value indicates a lower starting quantity of nucleic acid.

As previously reported, the Ct value correlates to the starting quantity of target mRNA [ 25].

Positive results were obtained with starting quantity as low as 0.1 viral copy.