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Seven fish were randomly added to each tank at the start of the exposure phase.
Appropriately shifting the start of the exposure, the temporal PSF can be directly obtained as begin{array}{*{20}l} i(t) &= int delta(s - t) ((delta otimes r)(s)) ds &= r(t), end{array} (5).
Moreover, because the observable range of the depth is limited to the width of the square wave, the start of the exposure is shifted to change the region of interest.
Mice were acclimated for 2 weeks before the start of the exposure studies.
The toxicants were dissolved in water and added to the terrain at the start of the exposure experiment.
They were seeded so that each culture dish contained about 10 cells at the start of the exposure.
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At the start of the exposures, a less concentrated Microcystis mixture collected from Pinto Lake during a bloom event (2.2 ppm [2,195 ppb] aqueous microcystin LR mixed with suspended Microcystis) was added to the low exposure tank (Tank 2).
Mice were acclimated for 11 18 days and were 5 8 weeks of age at the start of the exposures.
Water samples were collected at the start of the exposures (0 hr), and after 24 h (both before and after change of tank water) and after 48 h.
Immediately prior to the start of the exposures, glass chambers received 400 mL artificial water, prepared according to the ISO 7346 3:1996 guideline, containing 5 μg/L of 10 nm AgNP, 5 μg/L of Ag Bulk or 0.25 μg/L of silver nitrate.
Whole blood sampled two weeks prior to the start of the fish exposure and at week 12 of the study (the end of the fish exposure) was submitted to Veterinary Services at the University of Alaska Fairbanks for determination of clinical chemistry, hematology and evaluation of health.
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