The amount of reads identified is given at the overlap between CsaMLO8 and the start of the insertion in forward (TE start-F) and reverse (TE start-R) direction, at the overlap between the end of the insertion and CsaMLO8 in forward (TE end-F) and reverse (TE end-R) direction, and at the site of the insertion with only CsaMLO8 sequence in forward (WT-F) and reverse (WT-R) direction.
Thus, the inflammatory response is not a result of preparations before the start of the experiment (insertion of catheters, laparotomy, among other factors).
not determined (not relevant for this work) bGiven as the region from the His-tag to the start of candidate sequence insertion site, including the TEV site (7 aa) To introduce candidate genes into the panel of pCold-II vectors, megaprimers of each candidate were generated by PCR and then inserted into vectors by RF-cloning as described above.
The western showed that the AS11 DGAT1 was slightly larger (by 27 amino acids, probably due to the exon 2 repeat) than that of WT, while for the A7 DGAthe the largest possible stable transcript (truncated at the start of T-DNA insertion) encoded a protein that was significantly smaller than WT.
The time required for gastric tube insertion was measured with a stopwatch, from the time of starting transnasal insertion of the gastric tube to confirmation of successful placement.
Trials started with insertion of the lever into the chamber, and terminated with lever withdrawal.
As in the FIPP, trials started with insertion of the lever into the chamber, and terminated with lever withdrawal.
Tat protein has a potent SRS activity (see previous section), and probably it helps overcoming the mechanism just described that could be started by insertion of the viral genome with an upstream promoter near the 3′ ends.
Much has been written about how the degree of bow affects the starting point for insertion of the intramedullary rod and the accuracy of alignment of the varus/valgus osteotomy of the distal femur [ 17, 23, 24, 26, 28].
The immediate implant (IM) group and its CBD plotted against the time, starting at the time of insertion, is shown in Fig. 6.
A total of 45 patients were randomised to receive warfarin 1 mg daily started on the day of insertion and continued for a further 90 days or until development of clinical thrombosis.
