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Maximum adsorption MB dye was attained within 20 min after the start of every experiment.
p300 expression levels in each clone were determined at the start of every experiment.
All corals were acclimated to each experimental condition for 30 minutes before the start of every experiment.
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To further ensure that small differences in gain between CLEAr Lens channels were not responsible for observed differences in corneal potentials, a sine wave was introduced to all channels in the lens simultaneously at the start and conclusion of every experiment.
Before the start of the experiment, every lamellar edema found in the gills of fish was measured, and those that received natural feed had a higher branchial edema (p < 0.05).
At the start of the experiment, every subpopulation was inoculated with approximately 10 000 cells of O 9.
Blood samples were collected at the start of the experiment and every 2.5 weeks thereafter for 10 weeks.
The cultures were sampled for chemical analyses (pH, heavy metal concentration, and arsenic speciation) and estimation of colony forming unit (cfu) at the start of the experiment and every 7 days.
Nitrogen (N) and phosphorus (P) were added in a randomized factorial design using slow release fertilizer granules at the start of the experiment and repeated every 4 5 months for 2 years.
Blood samples were collected at day 0 before the start of the experiment and then every second week.
After the start of the experiment, TEER values were measured manually every 2 3 days using an epithelial voltohmmeter (EVOM) coupled to an Endohm-6 measurement chamber (World Precision Instruments, USA).
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