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In all experiments, mice were entrained to a 12∶12 LD cycle for at least one week prior to the start of all experiments (light intensity 36 µW/cm2≅120 lux).
At the start of all experiments the tip of a CASM was placed centrally on the top of the chosen neurone and moved down in 20 μm steps until there was a sudden fall in the potential, as shown in Fig. 1A.
Male wistar rats were allowed free access to a pelleted standard chow, containing 1.0% calcium and 0.65% phosphate and 4.5 IU Vitamin D3/g, and tap water and the animals were acclimated for 1 week prior to the start of all experiments.
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At the starting point of all experiments the age was 6-8 weeks.
Because the use of A2 allows for precise quantification of the endogenous TTR concentration in each plasma sample at the start of all subunit exchange experiments, these differences can be integrated into subsequent analyses of subunit exchange rates.
Prior to the start of experiments, all protocols were approved by the local independent ethical committee, according to Dutch law.
At the end of 12 weeks after the start of experiment, all rats were sacrificed and colons were evaluated for ACF. 10 mg/kg JTE522 significantly suppressed the total ACF/colon.
Before the start of the experiment, all fish were reared in a circular concrete tank with 5000 L well water and were fed a commercial diet for 2 weeks.
At the start of each experiment, all ferrets had body weights greater than 1.0 kg, with a range of 1.01 1.57 kg.
In order to minimize any effect of individual differences in stress reactivity to handling, prior to the start of the experiment all of the animals were handled for 90 sec/day, five days/week over a period of two weeks prior to the start of behavioral testing.
At the start of the experiment, all participants played one block of standard PGGs.
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