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A "hot start" method was used, and 1.25 U of Taq DNA polymerase (Sigma, St . Louis MO) was added after initial template denaturation.
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A forward stepwise method was used, starting with the factor that had the strongest association and adding other factors to the model one by one; those that were associated at P<0.05 were retained.
The parameters for running both methods were set similar to [ 20], that is, with QUASI-P a gradient decent method was used for optimization (start step size 0.01, stopping condition epsilon 0.01).
A mixture of commercial SiO and the carbon black powder with loose structures pretreated by an arc-discharge plasma method was used as the starting materials.
In this work, we successfully developed a novel wet chemical method to prepare LiNi0.5Mn1.5O4 coated LiMn2O4 (LMO@LNMO), in which commercial LiMn2O4 produced by solid state reaction method was used as the starting material and a nitrate precursor containing Li, Ni and Mn was used to form LiNi0.5Mn1.5O4 coating layer.
This sterilization method was used directly before the experiments starts.
This method was used by Euler.
This Note explains which method was used.
The same artifact rejection method was used.
As an alternative, one may choose a multistart strategy, where a local method is used repeatedly, starting from a number of different initial guesses for the parameters.
This method is used mainly in United States.
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