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PCR products of predicted length were cloned into the pGem-T easy plasmid vector (Promega) and sequenced using dye terminator cycle sequencing (DTCS Quick Start Kit, Beckman Coulter).
For in vitro folding, native protein was purified on Ni-NTA columns according to the manufacturer's specifications (Ni-NTA Fast Start Kit, QIAGEN).
The quantitative PCR reaction was carried out using the GenomeLabTM GeXP Start Kit (Beckman and Coulter) according to the protocol provided as described by [12].
After reverse transcription (GenomeLab™ GeXP Start Kit, Beckman-Coulter), a multiplex PCR reaction was conducted with Thermo-Start DNA Polymerase (ABgene).
Quantitative PCR were performed using the SYBR GREEN Jump Start kit (Sigma Aldrich) in duplicate on an ABI apparatus using standard procedure.
All sequencing was performed using the GenomeLab DTCS – Quick Start Kit (Beckman Coulter, High Wycombe, UK) according to the manufacturer's instructions, and a CEQ8000 automated sequencer (Beckman Coulter).
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The Sparkle Effect website offers a downloadable "Quick-Start" kit for squads across the country to implement a similar program.
Affinity chromatography was performed for enzyme purification as per the manufacturer's instructions (Ni-nitrilotriacetic acid [NTA] fast-start kit; Qiagen).
Recombinant proteins were purified by Ni-NTA Fast Start kit according to manufacturer's instruction (Qiagen).
His-CP25L was purified using the nickel-nitrilotriacetic acid (Ni-NTA) Fast Start kit (Qiagen).
The QIAexpress® Ni-NTA Fast Start Kit was used to purify 6×His-tagged protein (under naïve condition).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com