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The GC content profiles were computed by aligning all reads at their start coordinate (the − strand reads were reverse complemented) and counting the occurrences of the bases at each position starting from −2000 and ending at +2000 base pairs.
The alignment is presented in the following format: (H. bacteriophora EST start coordinate – H. bacteriophora EST end coordinate)/ H. bacteriophora length; (C. elegans start coordinate – C. elegans end coordinate)/ C. elegans length.
The distance is defined as: "start coordinate of the downstream copy" minus "end coordinate of the upstream copy".
The start coordinate and orientation of each gene was compared with those of its orthologs using custom perl scripts.
However, a penalty of -1 was given for each read that had the same start coordinate as another supporting read.
Using the CDS start coordinate, the novel transcript isoforms were translated and the position of the stop codon determined.
Similar(34)
Pretty soon, we figured, they would start coordinating their work hours with their coworkers.
The debt-ceiling vote next month and the budget debate after that promise more of the same — unless House and Senate Republicans stop bickering and start coordinating and talking differently.
The WHO has recommended the creation of an international network to conduct research, and the EU commissioner in charge of food has been asked to start coordinating a European response.
Using the WhatsApp messaging service on their cellphones, the local smugglers, who are tied in with networks of traffickers extending across West Africa, start coordinating the surreptitious loading of migrants from safe houses and basements across the city.
So get ready to start coordinating your fast days with family and friends; the 5 2 FastDiet is going to be around for awhile.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com