The PCR designs partially covered the coding regions, and therefore successful expression required recombination into a locus supplying the appropriate translational start context.
Therefore, although the predominant CPuORF start context (AAaa AUGa) seems to be weaker than the predominant mORF context, it remains to be determined whether ribosomes initiate efficiently at the uORF AUG.
Industrial networks and clusters have been proven to be one of the best models of local industrial development, and they can be considered also a favorable starting context for IS projects.
For starting context: Candy Crush, from December of 2013 to February of 2014 saw its DAUs rise to 97 million from 93, but its total games played (daily average) fell from 1.085 billion to 1.065 billion.
The monthly club night I started, Context, initially ran from January to December of 2004.
We think we have a head start on context but know that the other big players have similar end goals in mind.
The AUG start codon context, referred to as the Kozak motif, was reported as a consensus sequence for initiation of translation in vertebrates [16].
The AUG start codon context, also referred to as the Kozak sequence, is not strictly conserved in eukaryotes [ 30, 31].
AUG codons at the beginning of protein coding sequences are frequent in eukaryotic mRNAs with a suboptimal start codon context.
The start codon context adaptation index (AUGCAI) is 0.32, which is well within the range of other yeast mRNA (Miyasaka 1999; supplementary fig. S5A, Supplementary Material online).
