Sentence examples for start activation from inspiring English sources

Cycling conditions included a hot start activation (5 min, 95°C), 40 cycles of 10 sec denaturation (95°C), and annealing and extension (30 sec, 60°C).

The cycling reaction condition was: initial "Hot Start" activation step for 5 min at 94°C followed by 40 cycles of 30 s at 94°C, 30 s at 60°C, and 30 s at 72°C. 25 Each sample was detected in duplicate, and calculating the mtDNA content was based on the mean of Ct (cycles of threshold) values.

The temperature profile was thermal mixing at 70°C for 40 min and 60°C for 30 s, followed by hot start activation at 95°C for 60 s and 40 cycles of amplification (96°C for 5 s and 60°C for 20 s).

PCR thermocycling consisted of an RT step at 50°C for 30 min, hot start activation at 95°C for 3 min, followed by 40 amplification cycles of 95°C for 30 s, 52°C for 15 s, and 68°C for 1 min, with a final extension cycle at 68°C for 7 min. All PCR products were visualized after electrophoresis in 2% precast gels stained with ethidium bromide (Invitrogen) under UV illumination.

Our thermocycling conditions for fluorogenic one-step RT-PCR are: 35 minutes at 48°C (for reverse transcription; normally 30 minutes; ABI), 10 minutes at 95°C (for AmpliTaq Gold DNA polymerase hot-start activation), and 50 cycles of: 15 seconds at 95°C (for duplex melting), 1 minute at 58°C (for annealing and extension; normally 60°C; ABI).

The cycling parameters were as follows: 1 cycle of reverse transcription (30 min at 50°C), 1 cycle of Thermo-Start activation (12.5 min at 95°C), and 40 cycles of denaturation (15 s at 95°C) and annealing/extension (1 min at 60°C).

qPCR reactions (in duplicate) were performed using an Applied Biosystems 7300 Real-Time PCR System (Applied Biosystems, Carlsbad, CA) as follows: hot-start activation at 95°C for 2 min, 40 cycles of denaturation (95°C for 15 sec), and annealing/extension (55°C for 60 sec).

However, the studies in our review noted improvements across ranges of starting activation levels, suggesting that irrespective of baseline engagement levels, with the appropriate (personalized) support, increased engagement is both possible and beneficial.

The ship had been scheduled to arrive in San Diego by the end of the year to start the activation of its weapon system, the website reported.

Monster will start seeing activation from the endorser athletes within the coming weeks.

Reactions were incubated in a 96-well optical plate and the cycling began with template denaturation and hot start Taq activation at 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds and 60°C for one minute.