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The starch branching enzyme (2.4.1.18) acts on the linear glucans producing branched glucans [ 35].
The starch branching enzyme activity was calculated as the amount glucose-1-phosphate converted to branched starch per minute and μg total protein.
Starch branching enzyme.
Starch branching enzyme (SBE) introduces branch points into the amylopectin chains.
In addition, the metabolic step of ADP-glucose pyrophosphorylase is located upstream from that of starch synthase, starch branching enzyme, and starch debranching enzyme.
It specifically binds to the ACGT elements in the promoters of both granule-bound starch synthase (GBSS) and starch branching enzyme (SBE1) (Wang et al. 2013).
Modern evidence shows that an insertion of 800 nucleotides in the gene for starch branching enzyme (SBE1) renders that enzyme non-functional in the homozygous recessive (rr) condition.
The critical aggregation concentration (CAC) was measured for samples with different levels of OSA modification, of macromolecular size and degree of starch branching.
Potato tuber starch was genetically engineered in the plant by the simultaneous antisense suppression of the starch branching enzyme (SBE) I and II isoforms.
The latter (approximately 70 ~ 80% of starch) is a much larger molecule with frequent α-(1 6) branches formed by multiple enzymes, for example, starch synthases (SSs) and starch branching enzymes (SBEs) (Tetlow et al. 2004; Hannah and James 2008).
Starch is synthesized by four enzyme classes, with multiple subunits in each class: ADP-glucose pyrophosphorylase (AGPase); starch synthase (SS); starch branching enzyme (BE); and starch debranching enzyme (DBE).
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