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A series of peptides with various staple positions were investigated in detail.[ 419] Structural studies revealed significant differences in the binding mode, affinity, and selectivity.
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Staple placement was graded as adequate or inadequate depending on the location in relationship to the physis: incorrectly placed staples were not parallel to the physis, did not capture the physis, or had dislodged from the original position during the postoperative period.
In Figure 2, it is shown that the two double-staggered rows of staples stop at the 9.5 mark and only a single staple is positioned proximally.
This suggests that, as reported above with for VSV-G, anterograde transport of Collagen-I is not altered when staples are positioned within the Golgi.
Finally, ManII-GFP was co-expressed with DsRed-CD8lumenal, staples were positioned within the Golgi as explained above, and FRAP of ManII-GFP was performed at 20°C in the absence of the disaggregating drug (ManII-GFP [Stapled Golgi]).
Indeed, almost all of the peptides bearing a hydrocarbon staple at various positions were found to increase the binding affinity for PKA-RIIα.
Staple in place.
Expose the side of the staple with the prongs and position it facing up on the table.
Staple the ends into position.
Position the staple remover over the prongs of the staple and pinch the staple open.
These films were immensely popular with audiences, and were generally well received by critics, stapling Crawford's position as one of MGM's top female stars of the decade, along with Norma Shearer, Greta Garbo, and Jean Harlow.
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