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Two fatty acid standards were quantified in three randomly selected samples in both tests.
All C-labeled congener internal standards were quantified with isotope ratio errors less than 3%.
These standards were quantified under the assumption that steady-state metabolite levels observed in labeled and unlabeled extracts are equivalent.
Bottom, the optical densities (OD) of the bands corresponding to ParB-His6 standards were quantified (black circles) and were fit by a linear function (black line).
The resulting bands, corresponding to the RTA standards, were quantified by scanning laser desitometery, and a standard curve plotted (Fig. 4B).
Visualized lipids together with ergosterol, triolein and cholesteryl ester as standards were quantified by densitometric scanning at 400 and 600 nm using a Shimadzu scanner CS-930.
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The amount of free LF in the standards was quantified at 4.56 ng, which was 3%% of the total 150 ng LF loaded (Fig. 2a).
The peak areas of all the analytes, standards, blanks, and internal standard were quantified using Analyst 1.4.1 (MDS SCIEX, Ontario, Canada).
8‐Oxo‐7,8‐dihydro‐2′‐deoxyguanosine and its internal standard were quantified by LC‐MS/MS.
Lipids, expressed as ratios to the relevant internal standard, were quantified using standard curves prepared with sphingomyelin, galactosylceramide, and sulfatide external standards (Avanti Polar Lipids).
The optical density of the bands of the PCR products and the 300 bp standard were quantified by densitometry using Scion Image Software Betaa 4.02 Win; Scion Image).
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