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Fresh buffer and standards were prepared daily.
Standards were prepared with bovine serum albumin.
Sample dissolution and standards were prepared in identical matrixes.
All reagents and standards were prepared using double distilled water.
The test samples and standards were prepared in duplicate.
The working standards were prepared by serial volume/volume dilution in polypropylene vials (Sarstedt, Germany).
Silicic acid concentration standards were prepared with sodium metasilicate pentahydrate analysis.
Opium and opium alkaloids reference standards were prepared as previously described (The Japanese pharmacopoeia 2006).
Standards were prepared as serial 3-fold dilutions in quadruplicates.
BSA standards were prepared in 0.1 M perchloric acid.
T or P standards were prepared according to the manufacturer's instructions.
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