Sentence examples for standards were generated from inspiring English sources

Individual in-vitro transcribed RNA standards were generated for each sequence with methodology described earlier [36], and the paramyxovirus RNA concentrations in samples were determined.

Single-stranded poly-adenylated RNA standards were generated for TNF, IP-10, IκBα, IL1β and EF1α by in vitro transcription from the cloned DNA using T7 polymerase and were quantified by a Bioanalyzer (Agilent) and confirmed as a single species.

Synthetic RNA standards were generated by using T7 polymerase and linearized plasmid DNA.

Immunoglobulin standards were generated using purified human immunoglobulins of each isotype (Sigma, France).

Synthetic RNA standards were generated from linearized target sequence plasmids by using T7 polymerase (mMessage mMachine, Invitrogen).

Plasmid standards were generated by cloning amplified products into pCR2.1-TOPO vector (Life Technologies), then transformed into E. coli XL1 Blue (Agilent Technologies), extracted and purified.

Three images corresponding to the three samples (control, visceral leishmaniasis and internal standard) were generated for each gel.

From the purified cDNA, a 594 base pair ssRNA standard was generated using a MegaScript kit with TurboDNase treatment (Life Technologies).

The PtdIns 3,4,5 P3 standard was generated by phosphorylating PtdIns(4,5 P2 with recombinant human p110alpha in the presence of Pγ ATP.

Standard curves were generated using standard APPs supplied by the manufacturer (Dako, Ely, UK).

Standard curves were generated by quadruplicate cDNA standard.