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Accurate amounts of individual standards were added into a certain amount (0.1 g) of GL-1.
Surrogate standards were added to each sample to monitor the extraction performance and matrix effects.
The internal standards were added at the beginning of the sample preparation.
Surrogate standards were added to each sample to monitor extraction performance and matrix effects.
The isotope-labeled AQUA peptide standards were added to each sample at a constant amount (4 fmol).
Prior to the first step of extraction, several recovery standards (also called quality control standards) were added into the samples for quality control purposes.
Nicotine standards were added to the known sample (GP, batch no.7) at three different levels and then extraction and analysis were performed as described in experimental section.
In order to increase the accuracy of the analytical data, stable isotopically-labeled standards were added to the initial water samples.
Further 13C12-labelled internal standards were added to the extracts for the determination of the recovery of the internal standards added before.
Samples and standards were added to triplicate wells and incubated at 37°C for 2 h.
Briefly, plasma samples or E2 standards were added to the 96-well micro titer plates coated with mouse monoclonal antibody.
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