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The fluorescence threshold was set to 50 r.f.u. and the correct fit of size standards was checked for all electropherograms.
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Additionally, the standard recovery rates of 13C12-labelled quantification standards were checked.
The standard recovery rates of 13C12-labelled quantification standards were checked to be in the range of 50% to 150%.
Additionally, the standard recovery rates of 13C12-labelled quantification standards were checked to be in the range of 50% to 120%.
The validation of calibration curves constructed for elements present in the standards were checked through analysis of standard reference materials (Tuna fish for fish sample and Spinach for vegetable samples).
External calibration standards were analyzed with every set of samples, and recoveries of internal standards were checked against external calibration standards.
Genes retrieved by the selection of Clinical Management and Evidence Marker classes and not mentioned in the AD gold standard were checked manually by a biologist using the SCAIView environment.
Precision of the method, expressed as relative standard deviation, was checked at two levels obtaining good results (2.7 9.3%).
For M30 ELISA kit, the parallelism to the standard curve was checked with multiple dilutions of three bile samples: 1 2, 1 4, 1 8, 1 16.
This selection of Alu containing mRNAs was based upon detection of Alu repeats by Repeatmasker (http://www.repeatmasker.org), using standard settings, and was checked using the UCSC genome browser (http://genome.ucsc.edu/).ucsc.edu/
All recorded data were analysed using descriptive statistics (mean ± standard error) and normality was checked in all cases.
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