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Standards of glucose, fructose, sucrose and chicory inulin were used to calibrate the assay.
At a Rf between 0.1 and 0.2 there is mainly the sugars, glucose and xylose, out of the DES, which are comparable with the pure sugar standards of glucose (Gluc) and xylose (Xyl).
A calibration curve using a series of standards of glucose between 0 and 200 mg L−1 was constructed.
Figures 3(a)– 3 e) illustrated the results of HPLC analysis for monosaccharide standards of glucose, galactose, mannose, glucosamine, and N-acetylglucosamine, respectively, and Figure 3(f) revealed that for acidic hydrolytes of LUFFACHITIN as exemplified by HF-15, which was hydrolyzed in CF3COOH for 15 h.
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Hemoglobin A1c (HbA1c) is the standard measure of glucose control in persons with diagnosed diabetes mellitus and is now recommended for use as a diagnostic test for diabetes (1, 2).
Duplicate samples of the fermentation broths were analyzed and each analyte was quantified using a standard curve based on calibration curves of an external standard of glucose, xylose, mannose, galactose, arabinose.
For the separation of the aminoglycosides and the internal standard of glucose, a CarboPac™ PA1 anion exchange column was used together with a mobile phase consisting of a 0.070 mol L−1 sodium hydroxide solution in the isocratic elution mode with a flow rate of 0.8 mL min−1.
The comparator in these trials should be the current gold standard of glucose measurements in arterial blood by a blood gas analyser.
By comparing the retention times to a standard of glucose and reducing linear laminarioligos (G1 G7) we could clearly identify products resulting from hydrolytic activity (G2 and G3) as well as transferase activity (G5 G7, Fig. 4A).
Many authors recommend a PPBG level of <140 mg/dl 2 h after the start of a meal, to achieve normal glucose tolerance according to the standards of oral glucose tolerance testing.
These spots are identical with the standards of pure glucose (Rf 0.1) and xylose (Rf 0.16) which demonstrated that these spots are sugars of the sugar mix in the used DES.
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