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> Examples of the chromatograms of 18 standards obtained using UV-DAD detection with wavelength programming and fluorimetric detection as well as of a chromatogram of the PAH-rich fraction isolated from a sample of road asphalt 50/70 according to the procedure discussed in this paper obtained using UV-DAD and fluorimetric detection are shown in Fig. 4.
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The absorbance was measured at 520 nm and nitrite concentrations were calculated from a standard curve obtained using standards containing increasing concentration of NaNO2.
The purity of the sample was calculated from the standard curve obtained using standard P HB-co-HV) P HB-co-HVfrom Sigma-Aldrich, (USA) containing 12 mol% of HV.
The gold standards were obtained using the method described in [ 22].
Data were automatically processed and analyzed with Bio-Plex Manager Software 4.1 by using the standard curve obtained using a recombinant cytokine standard.
There is a limited correlation between the median IM values and the logarithmic standard deviations obtained using the two methodologies.
Cytokines were quantified based on standard curves obtained using an ELISA reader (Dynex, Chantilly, VA).
The reference standard was obtained using the triage nurse notes included on the surveillance records.
The amount of HB-EGF was estimated by the standard curve obtained using recombinant human HB-EGF.
The absorbance of the resulting extract was measured at 474 nm and converted to lycopene concentration (μg/mL) using a standard curve obtained using commercial lycopene (Sigma).
After dilution with distilled water (up to 5 ml), the A520 was taken, and the reducing power was evaluated using a standard curve obtained using maltose.
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