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During the experiments, the fish were observed daily, and every other day water quality was monitored for pH and NO2 with standards kits.
Superdex 200 pg 16/60 SEC columns (GE Healthcare) were calibrated with molecular weight standards from both the high and low molecular weight standards kits (GE Healthcare), and unknown protein weights were estimated using the manufacturers recommended protocol.
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Spectra were externally calibrated with Sequazyme™ Peptide Mass Standards Kit (ABI).
External calibration was performed using a mass standards kit for proteomics analyzer (Applied Biosystems).
The Superdex G200 column was calibrated with a molecular weight standards kit (Sigma).
An 8-point plate model external calibration was performed using the Sequazyme™ Peptide Mass Standards Kit (AB Sciex).
Employing recombinant (r) CK18 and caspase cleaved CK18 (CK18 Asp neo-epitope) as external standards, kit to kit reproducibly was <6% (n=19).
Default calibration was performed using the 4700 Proteomics Analyzer Standards Kit, while MS measurements were calibrated internally using trypsin and contaminant peaks (842.509, 2211.105, 2225.120 and 2807.315 Da).
There should have been more consultation with local people to assess their needs: "Beneficiary needs and capacities grew in their range and complexity with time, making the typical 'one size fits all' solution of standard kits or packs less appropriate".
Most businesses have standard kits they send out to their incoming interns.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com