The compounds represented by the peaks were putatively identified by comparison of HPLC retention times and spectral data against those of a standard extract (from beetroot, Figure 5A and 5E) and reported spectral data [ 14, 25, 26].
Two single donor high molecular weight mtDNA hg H (hg H*) and non-hg H (hg J2b1a) gDNA standards were extracted from peripheral blood from healthy West Eurasian donors using the QIAamp DNA Blood Maxi kit (Qiagen, Hilden, Germany), mtDNA control region sequence verified, and quantified according to [3].
The gold standards were extracted from the knock-out compendium elucidated by Rosetta (Hughes et al., 2000).
In brief, analytes and deuterated internal standards were extracted from 0.4 ml of human plasma using BondElut Certify solid-phase cartridges (Agilent Technologies, Santa Clara, CA, USA).
The analyte and the internal standard were extracted from plasma (900 μL) by liquid-liquid extraction using ethyl ether/hexane (50/50, v/v) and ammonium hydroxide (50%).
Briefly, MDZ, 1′-hydroxymidazolam and 1′-chlordiazepoxide (internal standard) were extracted from alkalinized (pH 9.5) plasma samples using liquid liquid extraction with 1-chlorobutane.
Analytes and internal standard were extracted from a 1-ml sample of EDTA-treated whole blood by liquid liquid extraction with 1-chlorobutane and separated with a YMC PDS AQ HPLC column (Waters, Milford, MA, USA).
Methotrexate and syringic acid (internal standard) were extracted from rat plasma samples by protein precipitation with acetonitrile.
The drug and internal standard was extracted from plasma with heptane isoamyl alcohol (95:5 v/v) and back extracted with sulfuric acid.
A 30-min standard EEG extracted from the continuous EEG recording is sent for centralised interpretation by a qualified neurophysiologist within 2 h after the initiation of continuous EEG monitoring.
