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The samples were diluted ten times using wash buffer and 25 μl of standards, control, and samples were pipetted into a 96-well plate in duplicate.
In brief, 50 μl each of the standards, control and serum samples were added to respective wells coated with estradiol (E2), follicle stimulating hormones (FSH), luteinizing hormones (LH) or progesterone (P4) antibody and incubated with 50 μl of enzyme conjugate for two hours at 37°C in oven (Echo Therm, USA).
Similar(58)
Ninety microlitres of standards, controls and samples were pipetted into duplicate wells in the bioassay plates.
To ascertain analytic quality all standards, controls and samples were analysed in duplicate and all duplicates with a CV >10% were reanalysed.
To ascertain analytic quality all standards, controls and samples were analyzed in duplicate and all duplicates with a coefficient of variation >10% were reanalyzed.
Standards, controls and samples were pipetted into the wells.
Measurements for standards, controls and serums were repeated for confirmation.
All standards, controls, and test samples were assayed in duplicate.
All of the standards, controls and samples were read in duplicate.
All of the standards, controls, and samples were run in triplicate.
All of the standards, controls, and samples were added to two wells of the microplate.
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