No one knows the gold standard beforehand, and even if the evaluation data is limited, it is well defined but still no fine tuning can be carried out.
We did not define acceptable variation in the definition of the reference standard beforehand.
Compound identification was based on: (i) comparison between retention times on an apolar column, and those of standards injected beforehand, and (ii) computer matching with commercial mass spectra libraries [ 53].
The resultant change in turbidity on precipitation of magnesium pyrophosphate in turn correlated with the CK19 mRNA copy number per microlitre of the original lysate via a standard curve established beforehand with three calibrators containing different CK19 mRNA copy numbers.
19 The resultant change in turbidity after precipitation of magnesium pyrophosphate was then correlated with the CK19 mRNA copy number per microlitre of the original lysate using a standard curve established beforehand using three calibrators containing different CK19 mRNA copy numbers.
The resultant change in turbidity on the precipitation of magnesium pyrophosphate was then correlated with the CK19 mRNA copy number per microlitre of the original lysate via a standard curve established beforehand using three calibrators containing different CK19 mRNA copy numbers.
The standard curve was drawn beforehand for determining the concentration of HCPT.
The standard curve was drawn beforehand for determining the FA concentration.
Most of these methods rely upon assuming that a discrete shift in rates at many sites has occurred over one branch in the protein phylogeny under examination and estimation of the phylogeny is usually performed beforehand using standard phylogenetic models.
The resulting change in turbidity upon precipitation of magnesium pyrophosphate was in turn correlated with CK19 mRNA copy number per μl of the original lysate by a standard curve, which was established beforehand with three calibrators containing different CK19 mRNA copy numbers.
