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PCR products were visualized by running on 1% agarose (Nusieve, FMC, Rockland, ME) gels containing SBYRgreen (Molecular Probes, Eugene, OR) at 1∶10,000 (5 µl in a 50 ml gel) and normalized against the housekeeping gene, β-actin, quantitated against standard curves using the same primers but based on standardized samples containing known copy numbers of cDNA, as previously described [20].
For standardized samples (n=12), ring molds were filled with mixed sealers based on epoxy resin, silicone, calcium hydroxide, zinc oxide-eugenol, glass ionomer, and polyketone.
The results suggest that proteomic experiments using more heterogeneous mouse samples would not require much larger sample sizes than those using narrowly standardized samples.
An area measuring 25 mm in diameter was delimited in the center of the wound to obtain standardized samples for structural and morphometric analysis.
Standardized samples of stool (200 μl) were added to 1 ml of diluent and centrifuged for 5 min at 12,300 rpm.
Protein standardized samples of CM were resolved in 10% SDS-PAGE containing 1 mg/ml gelatin (Sigma) and 1.5 μg/ml MMP-9 (Sigma).
Similar(51)
Taken together, high standards of sampling quality and standardized sample preparation allow reliable data to be generated even with long term storage or repeated freeze-thaw cycles.
There were scattered hot spots in species richness, suggesting the need for a directed and standardized sampling design.
While several studies have investigated invertebrate sampling methods [e.g. 15], [42] [44], there are still no generally accepted, standardized sampling methods or protocols for different invertebrate taxa.
Second, we carefully standardized sampling conditions so that PBL samples were drawn into RNA-stabilizing media and taken from both members of a twin pair at the same time and place.
Standardized sampling method allowed a spatial survey of contaminant levels and profiles.
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