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TSP assays were performed under standardized reaction conditions.
It was found that consistently reproducible amplification patterns were obtained only when rigorously optimized and standardized reaction conditions were employed.
Nevertheless, this promising approach suffers with a major limitation to date – a lack of a standardized reaction database to build and analyze metabolic network.
Substrates rac- 2a– 8a were subjected to enzymatic hydrolysis under standardized reaction conditions by using Pisa1, the eeP of sec-alcohols (S)- 2b– 8b formed was determined by GC analysis on a chiral stationary phase after extractive separation from the remaining non-hydrolyzed sulfate esters (S)- 2a– 8a.
With respect to AFM tip functionalization, this means that all (or almost all) benzaldehyde groups on the tip surface appear to react with a protein molecule under the standardized reaction conditions and that blocking of putatively unused benzaldehyde groups is merely optional.
Similar(55)
Quantification of results was accomplished by standardizing reactions to rpS17 levels, and then using the ΔΔCt method as described [45].
Standardized response means.
Getting advantage on the specimen type, Pandey et al. [ 53] and Silva et al. [ 54] standardized PCR reactions (snPCR and qPCR, respectively) to detect the parasite DNA from Giemsa-stained smears, prepared from bone marrow aspirates.
The model reaction when conducted at 80 °C with 10 mol % of SnO2 bulk led to prolonged reaction time with 72% yield (Table 1, Entry 7) which made us to standardize the reaction using SnO2 NPs.
The relative expression of the housekeeping gene was used for standardizing the reaction.
The transcript of actin gene was used to standardize each reaction run with respect to RNA integrity, sample loading and inter-PCR variations.
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