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Samples were collected using standardized phlebotomy procedures.
Ten ml of peripheral blood was drawn from the subjects using standardized phlebotomy procedures in BD Vacutainer spray-coated K2EDTA Tubes (BD, Franklin Lakes, NJ).
Using standardized phlebotomy procedures blood samples were collected and allowed to clot and then centrifuged at 2500 rpm for 10 min.
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The 30-ml syringe was primed with 4 ml of ACD-A before 26 ml of whole blood was obtained from each patient using standard phlebotomy procedures.
Plasma samples were collected according to standard phlebotomy procedures.
Blood was drawn using standard phlebotomy procedures and was collected without anticoagulant.
The nurse used standard phlebotomy procedures to obtain a fasting serum sample and a urine specimen.
The samples were collected according to standard phlebotomy procedures from consented patients.
For each participant, 2 ml of blood were collected from a peripheral vein into EDTA tubes (BD Vacutainer® Tubes, BD Vacutainer, Toronto) using standard phlebotomy procedures.
Participants were required to fast overnight prior to donating approximately 20 ml of venous blood from the antecubital vein using standard phlebotomy procedures.
Most study nurses were already trained in phlebotomy, however, phlebotomy procedures were taught locally by pathology laboratory trainers to those who needed it.
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