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The fatigue crack growth threshold, defining crack growth as either very slow or nonexistent, has been traditionally determined with standardized load reduction methodologies.
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To understand the cellular mechanism underlying bone defect healing in the context of tissue engineering, a reliable, reproducible, and standardized load-bearing large segmental bone defect model in small animals is indispensable.
Therefore, these elements are usually tested under standardized loading conditions in order to detect a sort of average material behaviour.
Total AKT (Cell Signalling #9272) was used to standardize loading.
GAPDH (Sigma-Aldrich) was used to standardize loading.
(1) Standardized loadings are determined by the loading and the variance of the items and latent variable, hence whilst unstandardized loadings are constrained to equality across groups, standardized loadings will differ; standardized loadings are shown for the white group.
Protein level of each lane was standardized by loading 30 µg of protein for every sample.
Note: SFL standardized factor loading, SMC squared multiple correlation, CR Construct reliability, AVE Average variance explained.
Figure 1 pretended the path diagram and standardized factor loading for each items.
Western blots were quantified by applying a Kodak gel documentation system (model 1D 3.5) and standardized with loading control.
Administration of the dual platelet inhibition was standardized; a loading dose was given 0 to 4 h before target temperature was reached and further maintenance doses were given every 12 (ticagrelor) or 24 (aspirin) h.
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