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CITE-Seq not only enabled to differentiate cellular subgroups based on surface protein expression, but also achieved a consistent output of protein detection with currently standardized flow cytometry.
Standardized flow cytometry was used to quantify T-cell subsets [ 18, 20] in laboratories participating in the National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID) AIDS Program Flow Cytometry Quality Assessment Program [ 21, 22].
For this purpose, droughts are characterized through a combination of relative standardized indices: Standardized Precipitation Index (rSPI), Standardized Precipitation Evapotranspiration Index (rSPEI) and a Standardized Flow Index (rSFI).
A spatiotemporal anisotropic semi-variogram is required to fully characterize the spatiotemporal variation of the standardized flow random field.
The spatiotemporal data structure of the standardized flow data, which are available at twelve stations for a period of 26 years (1975 2000), is demonstrated in Fig. 4.
Therefore, it is necessary to develop a spatiotemporal semi-variogram in order to fully characterize the spatiotemporal variation of the standardized flow random field.
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However, the standardized flows of different TDPs at different stations (for example, standardized flows of the first TDP at Station 1 and standardized flows of the second TDP at Station 3 may also be correlated.
In this regard, standardized flow-cytometric assessment of the major histocompatibility-II complex human leukocyte antigen (-D related) (HLA-DR) has gained particular interest.
The DAR method is shown to be quite robust; however, other methods of standardizing streamflow, such as standardizing flows by means (SM) or normalizing flows with means and standard deviations (SMS), are shown to be superior for a subset (∼42%) of sites.
The flow cytometer was standardized against Flow-set (Beckman Coulter, USA) fluorospheres.
It allows orientating agents towards adaptable but standardized information flow enabling interoperability in a loosely coupled manner.
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