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In practice, our method consists of a set of custom-made machined holders for standardized fabrication of polymeric stamps and for controlled alignment of successive printing steps.
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Early on, several guiding principles of synthetic biology were articulated, including design according to specification, separation of design from fabrication, use of standardized biological parts and organisms, and abstraction.
Template fabrication was similar to previous work.
And because conventional scaffold fabrication methods cannot realize the standardized 3D environment, we used micro-stereolithography (MSTL), which has the highest resolution among solid free-form fabrication (SFF) technologies that can produce scaffolds as designed.
Its fabrication relies on a fully standardized CMOS-like industrial process as well as on a single-step FSBM in post-process by means of TMAH anisotropic wet etching in order to release the micro-wire from the silicon bulk, which is well known to be a very competitive fabrication strategy.
Similar investments anchored energy-intensive microchip fabrication.
For the implementation of the masters suitable for cast molding fabrication, an inexpensive dry film photoresist, patternable using standard laboratory equipment, is benchmarked against standardized SU-8 masters obtained using UV lithography and systems manufacture in clean room facilities.
Development of a standardized procedure complying with professional codes and standards (RCC-MRx), a preliminary fabrication/welding procedure specification (pF/WPS), is described as well as a fabrication and characterization of feasibility mock-ups (FMU) aimed at assessing the suitability of a fabrication process for fulfilling the design and fabrication specifications.
The fabrication of microfluidic sensor chip and valve structure was performed by a standardized soft lithographic procedure [14, 16].
Total fabrication.
All fabrication.
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